Crest-derived cells isolated from chick gut migrate toward co-cultured netrin-1-expressing cells.
•Stably transfected cells expressing netrin-1 were embedded in 3-D collagen gels.  Immunoselected crest-derived cells were plated over the gels.
•Netrin-1 has a chemoattractive effect on immunoselected enteric crest-derived cells in vitro.