How do we know that regulatory factors travel
through the portal circulation to the pituitary (2)?

Harris and colleagues then transplanted the anterior
pituitary back under the midline hypothalamus, near the
portal vessels.  Normal endocrine function was restored,
and subsequent histology showed that the restoration of
function depended upon the successful revascularization
of the anterior pituitary by the primary capillary plexus of
portal vessels in the median eminence.
These experiments provided definitive proof of the
functional importance of the portal vascular system in
connecting hypothalamic regulation to anterior pituitary
function.